ELECTRICAL AND CHEMICAL EXCITABILITY APPEAR ONE WEEK BEFORE BIRTH IN THE EMBRYONIC RAT SPINAL-CORD

被引：70

作者：

MANDLER, RN ^{[1
]}

SCHAFFNER, AE ^{[1
]}

NOVOTNY, EA ^{[1
]}

LANGE, GD ^{[1
]}

SMITH, SV ^{[1
]}

BARKER, JL ^{[1
]}

机构：

[1] NINCDS,INSTRUMENTAT & COMP SECT,BETHESDA,MD 20892

来源：

BRAIN RESEARCH | 1990年 / 522卷 / 01期

关键词：

Embryogenesis; Flow cytometry; Kainic acid; Muscimol; Oxonal dye; Veratridine;

D O I：

10.1016/0006-8993(90)91575-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Embryonic rat spinal cord cells were acutely dissociated with the enzyme papain, stained with a voltage-sensitive oxonal dye and incubated with various pharmacological agents. Changes in the fluorescence intensity and, by inference, membrane potential of the cells were analyzed in a flow cytometer. Veratridine caused depolarization of the cells in a TTX-sensitive manner from as early as embryonic day 13. Depolarizing responses to muscimol and kainate appeared slightly later, at embryonic days 14 and 15, and were blocked by the antagonists bicuculline and CNQX, respectively. Responses to veratridine and kainate did not occur in sodium-free medium. The emergence of these excitable membrane properties coincides with postmitotic differentuation and synaptic development in the embryonic spinal cord. © 1990.

引用

页码：46 / 54

页数：9

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BENARI Y, IN PRESS J PHYSL LON

[2] EXPRESSION OF GABA AND GLYCINE RECEPTORS BY MESSENGER-RNAS FROM THE DEVELOPING RAT CEREBRAL-CORTEX [J].