DIRECT DETERMINATION OF LEAD IN HUMAN BLOOD AND SELENIUM, CADMIUM, COPPER, ZINC IN SERUM BY ELECTROTHERMAL ATOMIC-ABSORPTION SPECTROPHOTOMETRY USING ZEEMAN EFFECT BACKGROUND CORRECTION

The procedure for the direct determination of lead in human blood and selenium, cadmium, copper and zinc from human blood serum with micro quantities of the samples are described using electrothermal atomic absorption spectrophotometry with L'vov platform and Zeeman correction. No predigestion or extraction procedures are required. The analyses are performed by simple dilution of the specimen with Triton X-100 and/or proper matrix modifier. The method of standard additions and peak area measurement are used for quantitation. L'vov platforms have been used for all elements except cooper. Use of micro quantity of the sample, better reproducibility, low detection limit and possibility of analyzing a large number of samples per day made this technique particularly suitable as routine analytical procedure in clinical laboratory. The procedures for determination of the metal ions are applied on sixty normal persons and twenty-two cardiomyopathy patients to find any correlation. © 1990 Springer-Verlag.