PHOTOSENSITIZATION OF MCF-7 CELLS WITH DIAZIQUONE USING VISIBLE-LIGHT - CORRELATION WITH DNA STRAND BREAKS AND FREE-RADICAL PRODUCTION

被引：3

作者：

ALNABULSI, I ^{[1
]}

GUTIERREZ, PL ^{[1
]}

机构：

[1] UNIV MARYLAND,CTR CANC,DIV DEV THERAPEUT,655 W BALTIMORE ST,BALTIMORE,MD 21201

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1992年 / 56卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1992.tb02148.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of visible light to enhance the activity of diaziquone (AZQ) was evaluated in MCF-7 human breast cancer cells. MCF-7 cells were sensitive to AZQ, while visible light illumination had no appreciable effect on cell survival. In the presence of AZQ, visible light potentiated AZQ's cytotoxicity. This potentiation of AZQ activity correlated with a 2-2.5-fold increase in the formation of free radicals (hydroxyl radicals and AZQ semiquinone) and with the production of DNA strand breaks as measured by electron paramagnetic resonance and gel electrophoresis respectively. These results support the hypothesis that free radical formation is part of the mechanism of action of AZQ.

引用

页码：201 / 209

页数：9

共 40 条

[1] PHOTORADIATION THERAPY OF ENDOBRONCHIAL LUNG CANCERS EMPLOYING THE PHOTODYNAMIC-ACTION OF HEMATOPORPHYRIN DERIVATIVE [J].

BALCHUM, OJ ;

DOIRON, DR ;

HUTH, GC .

LASERS IN SURGERY AND MEDICINE, 1984, 4 (01) :13-30

[2] PHOTOINDUCED REACTIONS OF ANTHRAQUINONE ANTITUMOR AGENTS WITH PEPTIDES AND NUCLEIC-ACID BASES - AN ELECTRON-SPIN RESONANCE AND SPIN TRAPPING STUDY [J].

CARMICHAEL, AJ ;

RIESZ, P .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 237 (02) :433-444

[3] PHOTOGENERATION OF SUPEROXIDE BY ADRIAMYCIN AND DAUNOMYCIN - AN ELECTRON-SPIN RESONANCE AND SPIN TRAPPING STUDY [J].

CARMICHAEL, AJ ;

MOSSOBA, MM ;

RIESZ, P .

FEBS LETTERS, 1983, 164 (02) :401-405

[4]

DOUGHERTY T J, 1986, Seminars in Surgical Oncology, V2, P24, DOI 10.1002/ssu.2980020104

[5] TREATMENT OF CUTANEOUS T-CELL LYMPHOMA BY EXTRACORPOREAL PHOTOCHEMOTHERAPY - PRELIMINARY-RESULTS [J].

EDELSON, R ;

BERGER, C ;

GASPARRO, F ;

JEGASOTHY, B ;

HEALD, P ;

WINTROUB, B ;

VONDERHEID, E ;

KNOBLER, R ;

WOLFF, K ;

PLEWIG, G ;

MCKIERNAN, G ;

CHRISTIANSEN, I ;

OSTER, M ;

HONIGSMANN, H ;

WILFORD, H ;

KOKOSCHKA, E ;

REHLE, T ;

PEREZ, M ;

STINGL, G ;

LAROCHE, L .

NEW ENGLAND JOURNAL OF MEDICINE, 1987, 316 (06) :297-303

[6]

EGORIN MJ, 1985, CANCER RES, V45, P992

[7]

FINGAR VH, 1988, CANCER RES, V48, P3350

[8]

FINKELSTEIN E, 1979, MOL PHARMACOL, V16, P676

[9] THE REDUCTIVE METABOLISM OF DIAZIQUONE (AZQ) IN THE S9 FRACTION OF MCF-7 CELLS - FREE-RADICAL FORMATION AND NAD(P)H - QUINONE-ACCEPTOR OXIDOREDUCTASE (DT-DIAPHORASE) ACTIVITY [J].

FISHER, GR ;

GUTIERREZ, PL .

FREE RADICAL BIOLOGY AND MEDICINE, 1991, 10 (06) :359-370

[10] PHOTOSENSITIZED DEGRADATION OF DNA BY DAUNOMYCIN [J].

GRAY, PJ ;

PHILLIPS, DR ;

WEDD, AG .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1982, 36 (01) :49-57

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