TAU-ANTIGENIC CHANGES INDUCED BY GLUTAMATE IN RAT PRIMARY CULTURE MODEL - A BIOCHEMICAL APPROACH

被引：27

作者：

SAUTIERE, PE

SINDOU, P

COURATIER, P

HUGON, J

WATTEZ, A

DELACOURTE, A

机构：

[1] INSERM,U156,PL DE VERDUN,F-59045 LILLE,FRANCE

[2] FAC MED LIMOGES,HISTOL LAB,UNITE NEUROBIOL CELLULAIRE,LIMOGES,FRANCE

来源：

NEUROSCIENCE LETTERS | 1992年 / 140卷 / 02期

关键词：

TAU-PROTEIN; PRIMARY NEURONAL CULTURE; GLUTAMATE TOXICITY; NEURODEGENERATION; ALZHEIMERS DISEASE;

D O I：

10.1016/0304-3940(92)90104-F

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Primary neuronal cultures were treated with glutamate to induce an increase of Tau immunoreactivity similar to that observed in Alzheimer's disease. The Tau profile of neurones in culture before and after exposure to glutamate was analyzed on immunoblots with anti-Tau, anti-paired helical filaments (PHF) and antibody specific for modified Tau. Differences were observed between treated and control cultures: glutamate induced a shift of immunodetection from the lowest to the highest molecular weight Tau isoform and an acidification of Tau proteins. However, these modifications are not exactly those observed in Alzheimer's disease since we were not able to detect `Alzheimer-type' epitopes on Tau proteins after the glutamate exposure.

引用

页码：206 / 210

页数：5

共 24 条

[1] PATHOLOGICAL PROTEINS TAU-64 AND TAU-69 ARE SPECIFICALLY EXPRESSED IN THE SOMATODENDRITIC DOMAIN OF THE DEGENERATING CORTICAL-NEURONS DURING ALZHEIMERS-DISEASE - DEMONSTRATION WITH A PANEL OF ANTIBODIES AGAINST TAU-PROTEINS [J].