USE OF GENETICALLY-ENGINEERED LIPID-TAGGED ANTIBODY TO GENERATE FUNCTIONAL EUROPIUM CHELATE-LOADED LIPOSOMES - APPLICATION IN FLUOROIMMUNOASSAY

被引:20
作者
LAUKKANEN, ML
ORELLANA, A
KEINANEN, K
机构
[1] VTT Biotechnology and Food Research, FIN-02044 VTT Espoo
关键词
ANTIBODY ENGINEERING; EUROPIUM CHELATE; IMMUNOLIPOSOME; LANTHANIDE; LIPID-TAGGED ANTIBODY; TIME-RESOLVED FLUOROMETRY;
D O I
10.1016/0022-1759(95)00107-L
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Biosynthetically lipid-tagged single-chain antibody (Laukkanen et al., Protein Eng. 6 (1993) 449; Biochemistry 33 (1994) 11664) has been used to functionalize europium (Eu3+) chelate-loaded liposomes. The resulting Eu immunoliposomes displayed specific hapten-binding activity and little non-specific binding in time-resolved fluoroimmunoassay (TR-FIA). No loss of entrapped marker nor of binding activity was observed after storage of Eu immunoliposomes for 1 month at 4 degrees C. In comparison with Eu-labeled free single-chain antibody, Eu immunoliposomes produced a higher signal and provided increased sensitivity in a sandwich-type immunoassay. These results demonstrate the potential of Eu immunoliposomes as signal-amplifying reagents in TR-FIA.
引用
收藏
页码:95 / 102
页数:8
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