PARTIAL-PURIFICATION OF MEMBRANE-BOUND B AND C-CYTOCHROMES FROM CHROMATIUM-VINOSUM

Three membrane-bound cytochromes from the photosynthetic purple sulfur bacterium Chromatium vinosum have been partially separated from other membrane components by treatment with deoxycholate followed by ammonium sulfate fractionation and chromatography on Bio-Gel A1.5. Cytochrome c555, present in relatively small amounts in the deoxycholate extract, has an α-band that splits into two bands at 548 and 552.5 nm at liquid nitrogen temperature. The major components of the deoxycholate extract are cytochromes c553 and b560, which are present in essentially equimolar amounts through all the stages of the purification procedure. © 1979.