NONAMPHOTERIC ISOELECTRIC-FOCUSING .2. STABILITY OF BORATE-GLYCEROL PH GRADIENTS IN RECYCLING ISOELECTRIC-FOCUSING

被引：1

作者：

TODD, P ^{[1
]}

ELSASSER, W ^{[1
]}

机构：

[1] BIOPROC & PHARMACEUT RES CTR,PHILADELPHIA,PA

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 11期

关键词：

D O I：

10.1002/elps.1150111112

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

By complexing polyols with borate in recycling isoelectric focusing and by varying the ratio of polyol to borate over the useful pH range of 4.0‐6.0, it is possible to control pH. Twelve solutions of 0.1 M boric acid and varying glycerol concentration were used to vary pH in a twelve‐compartment commercial recycling isoelectric focusing (RIEF) system. Various concentrations of boric acid were tested as anolyte, and various Tris(hydroxymethylamino)methane‐borate buffer systems were tested as catholyte. Electroosmosis, hydrogen ion flow, and fluid balancing were characterized in two glycerol gradients; one was maintained at 0.06 pH/fraction and the other at 0.12 pH/fraction. In the latter case, ovalbumin (pI 4.70) migrated to the pH 4.61 and 4.72 compartments. It is concluded that the borate‐glycerol system can be adequately stabilized in RIEF for isoelectric purification of certain proteins. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：947 / 952

页数：6

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