ISOTYPE AND IMMUNOGLOBULIN SUBCLASS DISTRIBUTION OF EYE MUSCLE MEMBRANE REACTIVE ANTIBODIES IN THE SERUM OF PATIENTS WITH THYROID-ASSOCIATED OPHTHALMOPATHY AS DETECTED IN WESTERN BLOTTING

被引：4

作者：

BERNARD, NF

ERTUG, F

TEBOUL, N

ZHANG, ZG

SALVI, M

WALL, JR

机构：

[1] Thyroid Research Unit, Montreal General Hospital Research Institute, Montreal, QC, H3G IA4

来源：

AUTOIMMUNITY | 1991年 / 10卷 / 01期

关键词：

CYTOTOXICITY; EYE MUSCLE ANTIBODIES; THYROID-ASSOCIATED OPHTHALMOPATHY; WESTERN BLOTTING; 64 KDA PROTEIN;

D O I：

10.3109/08916939108997148

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have determined the immunoglobulin (Ig) class (isotype) and IgG subclass of autoantibodies in the serum of patients with thyroid-associated ophthalmopathy (TAO) or autoimmune thyroid disorders without evident ophthalmopathy reactive in Western blotting with antigens of 55, 64, 75 and 95 kDa in pig eye muscle membrane (PEMM). The 22 sera studied were shown, previously, to contain IgG antibodies reactive with one or more of the four antigens. The majority of sera antibodies reactive with PEMM antigens were of two or more IgG subclasses. of the IgG subclass specificities IgG1 and IgG2 subclass antibodies were, overall, the most common. We were unable to demonstrate IgG subclass restriction for antibodies reactive with the 95 or 55 kDa antigens in PEMM, antibody activity being equally distributed in all four subclasses tested. While most of the sera which recognized a 64 kDa antigen did so with an IgG4 antibody, all other subclasses were also represented. on the other hand all 13 sera reactive with a 75 kDa antigen did so using Ig of the IgG, subclass and 12 of these used the IgG, subclass as well, IgG, and IgG, subclasses being represented in only 3 and 4 sera, respectively. There were no differences, in respect to Ig class or IgG subclass distribution of eye muscle reactive antibodies between patients with Graves' hyperthyroidism with ophthalmopathy and those with Hashimoto's thyroiditis, and eye disease. Control sera from five normal subjects and three patients with non-autoimmune thyroid disorders did not contain antibodies reactive with these PEMM antigens of any Ig class or IgG subclass. IgG3 and IgG1 antibodies fix complement and can mediate cytotoxicity, while those of the IgG4 subclass do not. Antibodies to eye muscle membrane antigens thus have the potential to mediate cytotoxic damage of eye muscle cells, which is consistent with the hypothesis that one or more of these antibodies may be pathogenic in TAO. © 1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：57 / 63

页数：7

共 29 条

[1]

Salvi M., Zhang Z.-G., Haegert D., Woo M., Liberman A., Cadarso L., Wall J.R., Patients with endocrinc ophthalmopathy not associated with overt thyroid disease have multiple thyroid immunological abnormalities, J Clip Endocrinol Metab, 70, pp. 89-94, (1990)

[2]

Salvi M., Fukazawa H., Hiromatsu Y., Triller H., Bernard N., How J., Wall J.R., Role of autoantibodies in the pathogenesis of endocrine autoimmune disorders and in their association, Endocrine Reviews, 9, pp. 450-466, (1988)

[3]

Kodama K., Sikorska H., Bandy-Dafoe P., Baylcy R., Wall J.R., Demonstration of a circulating autoantibody against a soluble eye muscle antigen in Graves' ophthalmopathy, Lancet, 2, pp. 1353-1356, (1982)

[4]

Kendall-Taylor P., Atkinson S., Holcombe M., A specific IgG in Graves' ophthalmopathy and its relation to retro-orbital and thyroid autoimmunity, Brit Med J, 288, pp. 1183-1186, (1984)

[5]

Bemetz U., Moncayo R., Scherbaum W., Frisch H., Bordenhoop K., Schleusener H., Pfeifler E.F., Detection of IgG an IgM antibodies directed against eye and peripheral muscle prepatations in juvenile and adult patients with Graven' dixase and in patients with hyperthyroidism, (1986)

[6]

Mengistu M., Laryea E., Miller A., Wall J.R., Clinical significance of a new autoantibody against a human eye muscle soluble antigen detected by immunofluorescence, Clin Exp Immunol, 65, pp. 19-27, (1986)

[7]

Faryna M., Nauman T., Gordos A., Measurement of autoantibodies against human eye muscle plasma membrane in Graves' ophthalmopathy, Brit Med J, 290, pp. 191-192, (1985)

[8]

Hiromatsu Y., Fukazawa H., How J., Wall J.R., Antibody-dependent cell-mediated cytotoxicity against human eye muscle cells and orbital fibroblasts in Graves' ophthalmopathy - roles of class 11 MHC antigen expression and interferon-γ action on effector and target cells, Clin Exp Immunol, 70, pp. 593-603, (1987)

[9]

Wang P.W., Hiromatsu Y., Laryea E.A., Wosu L., How J.S., Wall J.R., Immunologically mediated cytotoxicity against human eye muscle cells in Graves' ophthalmopathy, J Clin Endocrinol Metab, 63, pp. 316-320, (1986)

[10]

Hiromatsu Y., Fukazawa H., Guinard F., Salvi M., How J., Wall J.R., A thyroid cytotoxic antibody that cross-reacts with an eye muscle cell surface antigen may be the cause of thyroid-associated ophthalmopathy, J. Clin Endocrinol Metab, 67, pp. 565-570, (1988)

← 1 2 3 →