ISOLATION OF A CDNA THAT ENCODES A NOVEL GRANULOCYTE N-FORMYL PEPTIDE RECEPTOR

被引：162

作者：

YE, RD

CAVANAGH, SL

QUEHENBERGER, O

PROSSNITZ, ER

COCHRANE, CG

机构：

[1] Department of Immunology, The Scripps Research Institute, La Jolla

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90629-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA of 1650 base pairs was isolated by screening an HL-60 granulocyte library with an N-formyl peptide receptor (NFPR) cDNA probe under low stringency conditions. The cDNA encodes a protein of 351 amino acids tentatively named FPR2, with a calculated molecular weight of 39 kDa. Sequence analysis revealed that FPR2 is 69% identical in sequence to the human NFPR and shares extensive homology to several other chemoattractant receptors. FPR2 expressed in transfected cells mediated formyl peptide-stimulated calcium mobilization at micromolar concentrations of ligand. FPR2 messenger is detected in granulocytic HL-60 cells, but not in undifferentiated HL-60 cells. These findings suggest that FPR2 is a novel receptor for formyl peptide ligand and a new member of the chemoattractant receptor gene family. © 1992.

引用

页码：582 / 589

页数：8

共 15 条

[1] SYNTHESIS AND USE OF A NOVEL N-FORMYL PEPTIDE DERIVATIVE TO ISOLATE A HUMAN N-FORMYL PEPTIDE RECEPTOR CDNA [J].