T-CELL SUBPOPULATION PHENOTYPES IN FILARIAL INFECTIONS - CD27 NEGATIVITY DEFINES A POPULATION GREATLY ENRICHED FOR T(H)2 CELLS

被引:28
作者
ELSON, LH
SHAW, S
VANLIER, RAW
NUTMAN, TB
机构
[1] NCI,EXPTL IMMUNOL LAB,BETHESDA,MD 20892
[2] NETHERLANDS RED CROSS,BLOOD TRANSFUS SERV,CENT LAB,AMSTERDAM,NETHERLANDS
关键词
CD27; FILARIASIS; IL-4; IL-5; LYMPHOCYTE MARKERS;
D O I
10.1093/intimm/6.7.1003
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Three-color flow cytometric analysis was used to define surface markers which identify the T(h)2-type CD4+ cells responsible for the eosinophilia and elevated serum IgE typical of tissue invasive helminth infections. A group of six mAbs to well known cell surface markers were screened for differential expression on CD4+ CD45RO+ lymphocytes from normal individuals (NL; n = 6) and filaria-infected patients (PT; n = 10). The majority of markers were expressed equally by both groups, but the CD4+ CD45RO+ cells in the PTs showed significantly higher levels of expression of HLA-DR than those of NLs (P = 0.014). This CD4+ HLA-DR+ subpopulation was then studied further for its expression of an additional 10 activation and adhesion molecules. CD27 showed a trend for lower intensities of expression on PT CD4+ HLA-DR+ cells than on those of NLs. Analysis of the serum from both NLs and PTs revealed that PTs had significantly higher levels of soluble CD27 and CD25 (IL-2R) in the serum than NLs (P < 0.01 and P = 0.022 respectively) indicating a general state of immune activation and differentiation. Functional analysis of the CD4+ HLA-DR+ and the CD4+ CD27- subpopulations revealed that the CD4+ HLA-DR+ cells produced significantly higher levels of IL-5 than the CD4+ HLA-DR- cells (P = 0.04), and the CD4+ CD27- cells produced significantly higher levels of both IL-4 and IL-5 than the CD4+ CD27+ cells (P <0.05 and P < 0.001 respectively). Thus, while the CD4+ CD27- and CD27+ subpopulations contain T(h)1 and T(h)O cells, only the CD4+ CD27- population contains the T(h)2 cells (producing both IL-4 and IL-5).
引用
收藏
页码:1003 / 1009
页数:7
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