DEVELOPMENT AND USE OF MONOCLONAL-ANTIBODY PROBES FOR IMMUNOHISTOCHEMISTRY, ELISA AND IFAT TO DETECT BACTERIAL AND PARASITIC FISH PATHOGENS

被引：39

作者：

ADAMS, A

THOMPSON, KD

MORRIS, D

FARIAS, C

CHEN, SC

机构：

[1] Institute of Aquaculture, Uniuersity of Stirling, Stirling, FK9 4LA, Scotland

来源：

FISH & SHELLFISH IMMUNOLOGY | 1995年 / 5卷 / 08期

关键词：

MONOCLONAL ANTIBODIES; DISEASE DIAGNOSIS; FISH VACCINES;

D O I：

10.1016/S1050-4648(95)80040-9

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The development of monoclonal antibody probes (MAbs) to bacterial and parasitic fish pathogens has provided useful tools for the rapid immunodiagnosis and control of disease. Immunohistochemistry, IFAT and ELISA tests have been developed to detect a variety of infectious agents, including those causing bacterial kidney disease (Renibacterium salmoninarum), mycobacteriosis (Mycobacterium spp.) and proliferative kidney disease (PKX). MAb probes are also being utilised as specific markers to identify and map potential vaccine candidates for PKD. As well as illustrating the invaluable contribution which MAbs can make to the control of fish diseases, the results indicate the ephemeral nature of some antigens and the importance of pathogen culture conditions prior to the production of MAbs. (C) 1995 Academic Press Limited

引用

页码：537 / 547

页数：11

共 27 条

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