MICROSCOPIC VISUALIZATION OF INSECT CELL BUBBLE INTERACTIONS .2. THE BUBBLE FILM AND BUBBLE RUPTURE

被引：82

作者：

CHALMERS, JJ ^{[1
]}

BAVARIAN, F ^{[1
]}

机构：

[1] OHIO STATE UNIV,DEPT CHEM ENGN,COLUMBUS,OH 43210

来源：

BIOTECHNOLOGY PROGRESS | 1991年 / 7卷 / 02期

关键词：

D O I：

10.1021/bp00008a010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this paper, the second in the series, the use of a microscopic, high-speed video system to study the interactions of two suspended insect cells strains, Trichoplusia ni (TN-368) and Spodoptera frugiperda (SF-9), with rupturing bubbles is reported. Events such as the adsorption of cells onto the bubble film and the mechanism of bubble rupture were observed. On the basis of these observations and the experimental and theoretical work of other researchers on bubble rupture and cell death as a result of sparging, it is proposed that cells are killed by the rapid acceleration of the bubble film after rupture and the high levels of shear stress in the boundary layer flow associated with bubble jet formation.

引用

页码：151 / 158

页数：8

共 23 条

[1] MICROSCOPIC VISUALIZATION OF INSECT CELL BUBBLE INTERACTIONS .1. RISING BUBBLES, AIR MEDIUM INTERFACE, AND THE FOAM LAYER [J].