CDP-CHOLINE ENTRAPMENT AND RELEASE FROM MULTILAMELLAR AND REVERSE-PHASE EVAPORATION LIPOSOMES

In this work it was reported the determination of CDP-choline entrapment efficiency in MLVs liposomes constituted of neutral or negatively charged phospholipids. The encapsulation capacity (EC) increases with using charged phospholipids; moreover, also the presence of cholesterol into the vesicle bilayers enhances the EC values. Liposomes, prepared with reverse-phase evaporation method, entrapped greater amounts of drug than multilamellar liposomes of the same composition. The size of liposomes so like as the polidispersity index values were determined by light scattering analysis. The rate of CDP-choline efflux from liposomes was determined 'in vitro'' and was dependent upon bilayer composition and the method of preparation.