ESTABLISHMENT OF PRIMARY EPITHELIAL-CELL CULTURE FROM ELUTRIATED RAT GASTRIC-MUCOSAL CELLS

被引：20

作者：

KINOSHITA, Y

HASSAN, S

NAKATA, H

ASAHARA, M

MATSUSHIMA, Y

KAWANAMI, C

PING, CY

MIN, D

NAKAMURA, A

CHIBA, T

机构：

[1] Division of Gerontology, Department of Medicine, Kobe University School of Medicine, Kobe, 650, 7-5-1 Kusunoki-cho, Chuo-ku

来源：

JOURNAL OF GASTROENTEROLOGY | 1995年 / 30卷 / 02期

关键词：

STOMACH; ISOLATED CELL; CELL CULTURE; GROWTH FACTORS;

D O I：

10.1007/BF02348656

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Proliferating cells in the gastric mucosal epithelium were successfully enriched by counterflow elutriation in a medium-sized cell fraction. When inoculated on culture plates coated with E-C-L cell attachment matrix, these cells differentiated into mucus-producing cells after reaching confluence. Northern blot analysis did not detect any transcript of the proton pump, histidine decarboxylase, somatostatin, or pepsinogen I, indicating the absence of parietal, ECL, D, and chief cells in the confluent monolayer. These mucus-producing cell monolayers that respond to various growth factors may be a suitable model with which to investigate the function of gastric mucus cells in vitro.

引用

页码：135 / 141

页数：7

共 25 条

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