ELECTRON-CAPTURE GAS-CHROMATOGRAPHY WITH SPLITLESS INJECTION ON ISOTHERMALLY OPERATED WIDE-BORE GLASS-CAPILLARY COLUMNS

The injection of large volumes (up to 50 μl) of diluted samples into a wide-bore isothermally operated glass capillary column has been studied. A series of n-alkanes (C11-C16) have been evluated as sample solvents by measurement of the resolution between the pesticides p,p′-DDD and o,p′-DDT. Variations in retention time for the solutes injected in different solvents and in differrent volumes of the same solvent have been measured. The effect on resolution of changing the injector port temperature has been studied at a constant column temperature of 210°. A double-injection technique utilizing a combination of a high-boiling (C15) and low-boiling (C16 n-alkane solvent is described. More than 500 injections with sample volumes of {slanted equal to or greater-than}5 μl have been made on a 0.77 mm I.D. SE-30 column without significant deterictation of its performance. © 1979.