MOLECULAR CHARACTERIZATION OF A PROTECTIVE, 11-KDA EXCRETORY-SECRETORY PROTEIN FROM THE PARASITIC STAGES OF TRICHOSTRONGLYLUS-COLUBRIFORMIS

被引：28

作者：

DOPHEIDE, TAA ^{[1
]}

TACHEDJIAN, M ^{[1
]}

PHILLIPS, C ^{[1
]}

FRENKEL, MJ ^{[1
]}

WAGLAND, BM ^{[1
]}

WARD, CW ^{[1
]}

机构：

[1] CSIRO,MCMASTER LAB,DIV ANIM HLTH,GLEBE,NSW 2037,AUSTRALIA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1991年 / 45卷 / 01期

关键词：

TRICHOSTRONGYLUS-COLUBRIFORMIS; EXCRETORY-SECRETORY; HOST PROTECTION;

D O I：

10.1016/0166-6851(91)90031-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An 11-kDa protein occurring as a major component of the non-glycosylated fraction of 4th larval stage (L4) and adult Trichostrongylus colubriformis excretory-secretory (ES) fluid has been found to be highly protective in guinea pigs, an alternate host for T. colubriformis. The protein has been purified, characterised and partly sequenced. With a reverse-complement oligonucleotide based on the carboxy-terminal sequence of the protein, recombinant lambda-gt11 clones were detected in an L4 cDNA library. The DNA sequence from one clone has a single extended open reading frame coding for a highly charged 11-kDa protein which lacks a leader sequence and contains a potential N-glycosylation site. Expression of the cloned DNA in Escherichia coli was detected with an antibody, raised in rabbits against gel-purified 11-kDa protein.

引用

页码：101 / 108

页数：8

共 26 条

[1] BASE-BASE MISMATCHES - THERMODYNAMICS OF DOUBLE HELIX FORMATION FOR DCA3XA3G + DCT3YT3G (X, Y = A,C,G,T)
ABOULELA, F
KOH, D
TINOCO, I
MARTIN, FH
[J]. NUCLEIC ACIDS RESEARCH, 1985, 13 (13) : 4811 - 4824
[2] BEFUS AD, 1986, VET PARASITOL, V20, P175
[3] BLOMSTROM DC, 1986, J BIOL CHEM, V261, P8811
[4] OESOPHAGOSTOMUM-RADIATUM - SUCCESSFUL VACCINATION OF CALVES WITH AN EXTRACT OF INVITRO CULTURED LARVAE
EAST, IJ
BERRIE, DA
FITZGERALD, CJ
[J]. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1988, 18 (01) : 125 - 127
[5] CHARACTERIZATION OF CDNA CLONES CODING FOR MUSCLE TROPOMYOSIN OF THE NEMATODE TRICHOSTRONGYLUS-COLUBRIFORMIS
FRENKEL, MJ
SAVIN, KW
BAKKER, RE
WARD, CW
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1989, 37 (02) : 191 - 199
[6] GRANT WN, IN PRESS INT J PARAS
[7] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[8] Maniatis T., 1982, MOL CLONING
[9] BASE-PAIRING INVOLVING DEOXYINOSINE - IMPLICATIONS FOR PROBE DESIGN
MARTIN, FH
CASTRO, MM
ABOULELA, F
TINOCO, I
[J]. NUCLEIC ACIDS RESEARCH, 1985, 13 (24) : 8927 - 8938
[10] VACCINATION AGAINST NEMATOSPIROIDES-DUBIUS IN MICE USING ADULT WORM EXTRACTS AS ANTIGENS
MONROY, F
BRINDLEY, PJ
DOBSON, C
[J]. AUSTRALIAN JOURNAL OF EXPERIMENTAL BIOLOGY AND MEDICAL SCIENCE, 1985, 63 (AUG): : 423 - 430

← 1 2 3 →