IMMUNOCYTOCHEMICAL LOCALIZATION OF RAT SUBSTANCE-P RECEPTOR IN THE STRIATUM

被引：128

作者：

SHIGEMOTO, R

NAKAYA, Y

NOMURA, S

OGAWAMEGURO, R

OHISHI, H

KANEKO, T

NAKANISHI, S

MIZUNO, N

机构：

[1] KYOTO UNIV, FAC MED, INST IMMUNOL, KYOTO 606, JAPAN

[2] KYOTO UNIV, COLL MED TECHNOL, KYOTO 606, JAPAN

来源：

NEUROSCIENCE LETTERS | 1993年 / 153卷 / 02期

关键词：

SUBSTANCE-P RECEPTOR; TACHYKININ; ANTIBODY; FUSION PROTEIN; STRIATUM; RAT; IMMUNOCYTOCHEMISTRY; ELECTRON MICROSCOPY;

D O I：

10.1016/0304-3940(93)90311-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A trp E fusion protein containing a C-terminal portion of the rat substance P receptor (SPR) was expressed in bacteria and used to produce an antibody. The antibody specifically reacted with SPR expressed in a mammalian cell line and rat striatum. Light and electron microscope analyses of the rat striatum revealed intense SPR-like immunoreactivity in neuronal somata and dendrites. These immunoreactive neurons constituted approximately 3% of the total population of striatal neurons; they were putative interneurons of large and medium-sized aspiny types.

引用

页码：157 / 160

页数：4

共 16 条

[1]

BOLAM JP, 1988, EXP BRAIN RES, V70, P361

[2] SUBSTANCE P-CONTAINING TERMINALS IN SYNAPTIC CONTACT WITH CHOLINERGIC NEURONS IN THE NEOSTRIATUM AND BASAL FOREBRAIN - A DOUBLE IMMUNOCYTOCHEMICAL STUDY IN THE RAT [J].