Plasma prolactin concentrations in the horse - Response to opioid receptor blockade with naloxone and comparison of two prolactin assay systems

被引:9
作者
Aurich, C
Burgmann, F
Hoppen, HO
Wuttke, W
Hoppe, H
Aurich, JE
机构
[1] HANNOVER SCH VET MED,GEBURTSHILFE & GYNAKOL RINDES KLIN,W-3000 HANNOVER,GERMANY
[2] HANNOVER SCH VET MED,ABT ENDOKRINOL,W-3000 HANNOVER,GERMANY
[3] BRANDENBURG HAUPT & LANDGESTUT,NEUSTADT,GERMANY
[4] UNIV GOTTINGEN,FRAUENKLIN,ABT KLIN & EXPTL ENDOKRINOL,W-3400 GOTTINGEN,GERMANY
关键词
D O I
10.1111/j.1439-0531.1995.tb00625.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The aim of this study was to investigate an involvement of endogenous opioids in the regulation of prolactin release in the horse. Effects of the opioid antagonist naloxone on plasma prolactin concentrations were determined and prolactin was measured with a homologous radio-immunoassay and an immunometric enzyme assay utilizing a combination of two monoclonal antibodies. Both assays were sensitive and specific for prolactin determination in the horse. However, low prolactin concentrations could be better differentiated with the radio-immunoassay. Experiments in stallions were performed in May, August and December, and in mares between April and June. Naloxone (approximate to 0.5 mg/kg i.v.) stimulated prolactin release in stallions. Prolactin secretion determined by radio-immunoassay in stallions receiving naloxone was significantly higher than in controls in May (p < 0.05) and August (p < 0.05), and nearly reached statistical significance in December (p = 0.06). Prolactin release measured by enzyme immunoassay was significantly different (p < 0.05) between naloxone and control experiments at all times. Endogenous opioids therefore inhibit prolactin release in stallions. The activation of these opioid systems is affected by season. Opioid-receptor blockade with naloxone did not influence prolactin release in mares.
引用
收藏
页码:279 / 287
页数:9
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