MODULATION OF CALCIUM-ACTIVATED NONSELECTIVE CATION CHANNEL ACTIVITY BY NITRIC-OXIDE IN RAT BROWN ADIPOSE-TISSUE

被引:34
作者
KOIVISTO, A
NEDERGAARD, J
机构
[1] Wenner-Gren Institute, Arrhenius Laboratories F3, Stockholm University
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1995年 / 486卷 / 01期
关键词
D O I
10.1113/jphysiol.1995.sp020790
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Single-channel calcium-activated non-selective cation currents from isolated rat brown fat cells were measured using the inside-out patch configuration of the patch-clamp technique. The existence of a possible modulatory effect of nitric oxide on the putative redox-modulatory site located on the intracellular side of the non-selective cation channel was investigated. 2. The nitric oxide-releasing substances nitroglycerin, sodium nitroprusside, S-nitrosocysteine and S-nitroso-N-acetyl-D,L-penicillamine (all at 100 mu M) were able to block channel activity almost completely. 3. In each case the blockade was persistent and could not be washed away. Dithiothreitol (DTT, 2 mM) was able to reverse the blockade to a large extent, whereas oxidized DTT (2 mM) was without effect. 4. It was concluded that nitric oxide can modulate non-selective cation channel activity by oxidizing sulfhydryl groups and that this effect can be reversed by reduction.
引用
收藏
页码:59 / 65
页数:7
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