ISOLATION AND STRUCTURE OF AMINO-TERMINAL CROSS-LINKING REGION IN INSOLUBLE TYPE-3 COLLAGEN

被引：13

作者：

BECKER, U ^{[1
]}

FIETZEK, PP ^{[1
]}

NOWACK, H ^{[1
]}

TIMPL, R ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM, BINDEGEWEBS FORSCH ABT, D-8033 MARTINSRIED, FED REP GER

来源：

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE | 1976年 / 357卷 / 10期

关键词：

D O I：

10.1515/bchm2.1976.357.2.1409

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A collagenous peptide T1X was isolated from a tryptic digest of the insoluble matrix of calf skin. The peptide consists of 2 identical polypeptide chains each with a length of 72 amino acid residues joined by a cross-link. Absorption spectra obtained from hydrazone and azine derivatives of T1X indicated that the peptide contains an aldol-type of cross-link (X). The sequence of 23 amino acid residues in the amino-terminal region was determined as Glx-Tyr-Glu-Ala-Tyr-Asp-Val-X-Ser-Gly-Val-Ala-Gly-Gly-Gly-Ile-Ala-Gly-Tyr-Hyp-Gly-Pro-Ala. This sequence overlaps the previously described amino-terminal sequence of .alpha.1 (III) chain obtained from pepsin treated, insoluble type III collagen. The present data demonstrate a nonhelical segment of 14 amino acid residues in type III collagen important for cross-linking.

引用

页码：1409 / 1415

页数：7

共 30 条

[1] CHEMISTRY OF COLLAGEN CROSS-LINKS - NATURE OF CROSS-LINKS IN POLYMORPHIC FORMS OF DERMAL COLLAGEN DURING DEVELOPMENT [J].

BAILEY, AJ ;

SIMS, TJ .

BIOCHEMICAL JOURNAL, 1976, 153 (02) :211-&

[2] TRYPTIC PEPTIDES FROM CROSSLINKING REGIONS OF INSOLUBLE CALF SKIN COLLAGEN [J].

BECKER, U ;

FURTHMAYER, H ;

TIMPL, R .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1975, 356 (01) :21-32

[3]

BECKER U, 1976, IMMUNOLOGY, V31, P57

[4] NATURE OF INTRAMOLECULAR CROSS-LINKS IN COLLAGEN . SEPARATION AND CHARACTERIZATION OF PEPTIDES FROM CROSS-LINK REGION OF RAT SKIN COLLAGEN [J].

BORNSTEI.P ;

PIEZ, KA .

BIOCHEMISTRY, 1966, 5 (11) :3460-&

[5] LIMITED CLEAVAGE OF NATIVE COLLAGEN WITH CHYMOTRYPSIN TRYPSIN AND CYANOGEN BROMIDE [J].

BORNSTEIN, P ;

KANG, AH ;

PIEZ, KA .

BIOCHEMISTRY, 1966, 5 (12) :3803-+

[6] A REINVESTIGATION OF HYDRAZINOLYTIC PROCEDURE FOR DETERMINATION OF C-TERMINAL AMINO ACIDS [J].

BRAUN, V ;

SCHROEDER, WA .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1967, 118 (01) :241-+

[7] COLLAGEN POLYMORPHISM - CHARACTERIZATION OF MOLECULES WITH CHAIN COMPOSITION [ALPHA-1(III)]3 IN HUMAN TISSUES [J].

CHUNG, E ;

MILLER, EJ .

SCIENCE, 1974, 183 (4130) :1200-1201

[8]

EDMAN P, 1970, PROTEIN SEQUENCE DET, P211

[9]

EPSTEIN EH, 1974, J BIOL CHEM, V249, P3225

[10]

EPSTEIN EH, 1975, J BIOL CHEM, V250, P9304

← 1 2 3 →