CONVENIENT QUANTITATIVE SYNTHESIS OF METHYLGLYOXAL FOR GLYOXALASE I ASSAYS

被引：61

作者：

KELLUM, MW ^{[1
]}

ORAY, B ^{[1
]}

NORTON, SJ ^{[1
]}

机构：

[1] N TEXAS STATE UNIV,DEPT BASIC HLTH SCI,DENTON,TX 76203

来源：

ANALYTICAL BIOCHEMISTRY | 1978年 / 85卷 / 02期

关键词：

D O I：

10.1016/0003-2697(78)90258-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, convenient, and quantitative method for the preparation of methylglyoxal (pyruvaldehyde) solutions is described. The method involved acid hydrolysis (5% H2SO4) of pyruvaldehyde dimethyl acetal at 100°C for 25 min. The hydrolysis method is quite reproducible and does not require standardization of the methylglyoxal solutions. The preparations of methylglyoxal by the procedure described have been used in the study of glyoxalase I activity. © 1978.

引用

页码：586 / 590

页数：5

共 15 条

[1] RAPID ASSAY FOR GLYOXALASE ENZYME SYSTEM [J].

ALEXANDE.NM ;

BOYER, JL .

ANALYTICAL BIOCHEMISTRY, 1971, 41 (01) :29-&

[2]

Dakin HD, 1913, J BIOL CHEM, V14, P155

[3]

Friedemann TE, 1927, J BIOL CHEM, V73, P331

[4] ON THE DISTRIBUTION OF GLYOXALASE AND GLUTATHIONE [J].

HOPKINS, FG ;

MORGAN, EJ .

BIOCHEMICAL JOURNAL, 1945, 39 (04) :320-324

[5] The glyoxalase activity of tissues. [J].

Jowett, M ;

Quastel, JH .

BIOCHEMICAL JOURNAL, 1934, 28 :162-172

[6]

KLOTZSCH H, 1965, METHOD ENZYMAT AN, P283

[7] 110 The polymerisation of methylglyoxal [J].

Moulds, LDV ;

Riley, HL .

JOURNAL OF THE CHEMICAL SOCIETY, 1938, :621-626

[8]

Neuberg C, 1913, BIOCHEM Z, V49, P502

[9] 2-STEP PURIFICATION OF MOUSE-LIVER GLYOXALASE-I AND EVIDENCE OF ITS DIMERIC CONSTITUTION [J].

ORAY, B ;

NORTON, SJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 483 (01) :203-209

[10]

Platt ME, 1934, J BIOL CHEM, V106, P179

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