AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS STRUCTURAL PROTEINS COMPARED FROM INVIVO AND INVITRO SOURCES

被引：19

作者：

MARUNIAK, JE

SUMMERS, MD

FALCON, LA

SMITH, GE

机构：

[1] TEXAS A&M UNIV,TEXAS AGR EXPT STN,DEPT ENTOMOL,COLLEGE STN,TX 77843

[2] UNIV CALIF BERKELEY,DEPT ENTOMOL SCI,BERKELEY,CA 94720

来源：

INTERVIROLOGY | 1979年 / 11卷 / 02期

关键词：

Autographa californica; Comparison; Proteins;

D O I：

10.1159/000149017

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Polyhedrin obtained from Autographa californica nuclear polyhedrosis virus (AcA/NPV) was apparently not modified in terms of primary structure after passage through alternate host systems, both in vivo and in vitro, as investigated by two-dimensional, high-voltage electrophoresis of tryptic digests of this protein as well as amino acid analysis. N-terminal analyses were conducted using an improved technique which showed proline to be the N-terminal amino acid. In addition, passage of enveloped nucleocapsids through alternate hosts as studied by SDS-PAGE showed the polypeptide compositions to be very similar. © 1979 S. Karger AG, Basel.

引用

页码：82 / 88

页数：7

共 20 条

[1] BIOCHEMICAL COMPARISON OF POLYHEDRAL PROTEIN FROM 5 NUCLEAR POLYHEDROSIS VIRUSES INFECTING PLUSIINE LARVAE (LEPIDOPTERA-NOCTUIDAE) [J].