REGULATION OF ENZYMES INVOLVED IN BIOSYNTHESIS OF 2,3-DIHYDROXYBENZOIC ACID IN AEROBACTER AEROGENES AND ESCHERICHIA COLI

被引:71
作者
YOUNG, IG
GIBSON, F
机构
关键词
D O I
10.1016/0304-4165(69)90302-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Assays for the enzymes in Aerobacter aerogenes converting chorismate into 2,3-dihydroxybenzoate (isochorismate synthetase, 2,3-dihydro-2,3-dihydroxybenzoate synthetase and 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase) have been developed. Isochorismate synthetase is stimulated by Mg2+ and 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase requires NAD for activity. Isochorismate synthetase has been separated from the other two enzymes by column chromatography on DEAE-cellulose. The addition of iron to the culture medium of A. aerogenes represses each of the enzymes. An experiment with a multiple aromatic auxotroph of Escherichia coli indicates that iron alone, not complexed with any 2,3-dihydroxybenzoate derivative, represses the enzymes concerned with 2,3-dihydroxybenzoate synthesis. © 1969.
引用
收藏
页码:401 / &
相关论文
共 21 条