COMPARATIVE-ASSESSMENT OF THE SPECIFICITY OF THE BRINE SHRIMP AND MICROTOX ASSAYS TO HEPATOTOXIC (MICROCYSTIN-LR-CONTAINING) CYANOBACTERIA

被引：81

作者：

CAMPBELL, DL ^{[1
]}

LAWTON, LA ^{[1
]}

BEATTIE, KA ^{[1
]}

CODD, GA ^{[1
]}

机构：

[1] UNIV DUNDEE,DEPT BIOL SCI,DUNDEE DD1 4HN,SCOTLAND

来源：

ENVIRONMENTAL TOXICOLOGY AND WATER QUALITY | 1994年 / 9卷 / 01期

关键词：

D O I：

10.1002/tox.2530090109

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Specific, straightforward, and rapid procedures are required for the detection, identification, and quantification of the potent low molecular weight toxins that are produced by blooms and scums of cyanobacteria (blue-green algae) in waterbodies. Use of the Microtox bioluminescence assay and the brine shrimp (Artemia salina) has been advocated for the initial screening of cyanobacterial blooms for microcystin hepatotoxins. Inhibition of bacterial luminescence in the Microtox assay and brine shrimp mortality were determined with microcystin-containing and nonmicrocystin-containing cyanobacteria. Extraction and fractionation of test samples was undertaken to select and isolate microcystin-containing fractions and reduce interference from other fractions. Maximal inhibition of bacterial luminescence in the Microtox assay occurred with fractions from Microcystis strains and an Anabaena bloom that did not contain microcystins. By contrast, the bioassay of fractions using brine shrimps correlated with the distribution of microcystin-LR in the fractionated Microcystis extracts. (C) 1994 by John Wiley & Sons, Inc.

引用

页码：71 / 77

页数：7

共 31 条

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