STUDIES ON THE EFFECT OF NAD (H) ON NITROGENASE ACTIVITY IN RHODOSPIRILLUM-RUBRUM

被引:16
作者
SOLIMAN, A [1 ]
NORDLUND, S [1 ]
机构
[1] UNIV STOCKHOLM, ARRHENIUS LAB, DEPT BIOCHEM, S-10691 STOCKHOLM, SWEDEN
关键词
NITROGEN FIXATION; NITROGENASE; SWITCH-OFF; METABOLIC REGULATION; RHODOSPIRILLUM-RUBRUM;
D O I
10.1007/BF00249100
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The effect of NAD(P) and analogs of this nucleotide on nitrogenase activity in Rhodospirillum rubrum has been studied. Addition of NAD+ to nitrogen fixing Rsp. rubrum leads to inhibition of nitrogenase. NADP+ has the same effect but NADH or analogs modified in the nicotinamide portion do not cause inhibition. In contrast to ammonium ions, addition of NAD+ leads to inhibition of nitrogenase in cells that have been N-starved under argon. The inhibitory effect of NAD+ is more pronounced at lower light intensities. Addition of NAD+ also leads to inhibition of glutamine synthetase, a phenomenon also occurring when "switch-off" is produced by the addition of effectors such as ammonium ions or glutamine. It is also shown that NAD+ is taken up by Rsp. rubrum cells.
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页码:431 / 435
页数:5
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