INHIBITION OF PHOSPHOLIPASE-A2 PURIFIED FROM HUMAN HERNIATED DISK

被引：12

作者：

CARABAZA, A ^{[1
]}

CABRE, F ^{[1
]}

GARCIA, AM ^{[1
]}

GOMEZ, M ^{[1
]}

SANCHEZ, J ^{[1
]}

MAULEON, D ^{[1
]}

CARGANICO, G ^{[1
]}

机构：

[1] LABS MENARINI SA, DEPT R&D, ALFONSO XII 587, E-08912 BADALONA, SPAIN

来源：

BIOCHEMICAL PHARMACOLOGY | 1993年 / 45卷 / 03期

关键词：

D O I：

10.1016/0006-2952(93)90158-S

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect on human herniated intervertebral disc phospholipase A2 (HD-PLA2) of a number of retinoids, antirheumatic drugs and reported PLA2 inhibitors was evaluated using autoclaved [1-C-14]oleate-labeled Escherichia coli membranes as the substrate. Dexamethasone, non-steroidal antiinflammatory drugs, aristolochic acid and retinol were inactive, whereas a marked inhibition was found for manoalide, retinal, nordihydroguaiaretic acid and p-bromophenacyl bromide after preincubation with the enzyme (IC50 values 0.25, 4, 5 and 5 muM, respectively). The results are parallel to those obtained with the PLA2 purified from human synovial fluid.

引用

页码：783 / 786

页数：4

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