PURIFICATION AND CHARACTERIZATION OF VITELLIN FROM THE MATURE OVARIES OF PRAWN, PENAEUS-MONODON

被引：53

作者：

CHANG, CF

LEE, FY

HUANG, YS

机构：

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1993年 / 105卷 / 02期

关键词：

D O I：

10.1016/0305-0491(93)90249-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. A purified vitellin was isolated from the mature ovaries of the prawn (Penaeus monodon) after purification with gel filtration, hydroxylapatite, DEAE-Sephacel and polyacrylamide gel electrophoresis. 2. Eight polypeptide subunits and a molecular weight of 492 kDa were suggested as being present in the purified vitellin. 3. Polypeptide subunits are associated with each other through non-disulfide bonds, and the subunits of the purified vitellin were easily rearranged during the purification. 4. It has been suggested that the purified vitellin is a lipo-glyco-carotenoprotein. The amino acid composition of the vitellin has also been analyzed.

引用

页码：409 / 414

页数：6

共 20 条

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