OVEREXPRESSION OF THE BCL-2 PROTEIN INCREASES THE HALF-LIFE OF P21(BAX)

被引:110
作者
MIYASHITA, T
KITADA, S
KRAJEWSKI, S
HORNE, WA
DELIA, D
REED, JC
机构
[1] LA JOLLA CANC RES FDN,LA JOLLA,CA 92037
[2] IDUN PHARMACEUT INC,SAN DIEGO,CA 92121
[3] IST NAZL TUMORI,DIV OSA,I-20133 MILAN,ITALY
关键词
D O I
10.1074/jbc.270.44.26049
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bcl-2 and Bax are homologous proteins which can heterodimerize with each other, These proteins have opposing effects on cell survival when overexpressed in cells, with Bcl-2 blocking and Bax promoting apoptosis, Here we demonstrate that gene transfer-mediated elevations in Bcl-2 protein levels result in a marked increase in the steady state levels of endogenous p21(Bax) protein as determined by immunoblotting in the Jurkat T-cell and 697 pre-B-cell leukemia cell lines, but not in several other cell lines including CEM T-cell leukemia, 32D.3 myeloid progenitor, PC12 pheochromocytoma, and NIH-3T3 fibroblasts. Steady-state levels of p21(Bax) protein were also elevated in the lymph nodes of Bcl-2 transgenic mice in which a BCL-2 transgene is expressed at high levels in B-cells. Northern blot analysis of BCL-2-transfected and control-transfected Jurkat and 697 leukemia cells revealed no Bcl-2-induced alterations in the steady-state levels of BAX mRNAs, In contrast, L-[S-35]methionine pulse-chase analysis indicated a marked increase in the half-life (t(1/2)) of the p21(Bax) protein in BCL-2-transfected 697 cells compared to control-transfected cells (t(1/2) > 24 h versus similar to 4 h), whereas the rate of Bax degradation was unaltered in Bcl-2-transfected CEM cells, The results demonstrate that levels of the proapoptotic p21(Bax) protein can be post-translationally regulated by Bcl-2, probably in a tissue-specific fashion, and suggest the existence of a feedback mechanism that may help to maintain the ratio of Bcl-2 to Bax protein in physiologically appropriate ranges.
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页码:26049 / 26052
页数:4
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