THE JELLYFISH GREEN FLUORESCENT PROTEIN - A NEW TOOL FOR STUDYING ION-CHANNEL EXPRESSION AND FUNCTION

被引：219

作者：

MARSHALL, J

MOLLOY, R

MOSS, GWJ

HOWE, JR

HUGHES, TE

机构：

[1] YALE UNIV,SCH MED,NEUROBIOL SECT,NEW HAVEN,CT 06520

[2] YALE UNIV,SCH MED,DEPT OPHTHALMOL & VISUAL SCI,NEW HAVEN,CT 06520

来源：

NEURON | 1995年 / 14卷 / 02期

关键词：

D O I：

10.1016/0896-6273(95)90279-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Two methods are described for using the jellyfish green fluorescent protein (GFB) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing.

引用

页码：211 / 215

页数：5

共 16 条

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