EFFECT OF VERAPAMIL ON THE CALCIUM AND MAGNESIUM TRANSPORTS OF RAT-KIDNEY CORTEX MITOCHONDRIA

被引：15

作者：

KOHDA, C

GEMBA, M

机构：

[1] Department of Pharmacology, Osaka College of Pharmacy, Kawai

来源：

JAPANESE JOURNAL OF PHARMACOLOGY | 1979年 / 29卷 / 05期

关键词：

D O I：

10.1254/jjp.29.745

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of verapamil on Ca2+ and Mg2+ accumulation was investigated in isolated rat kidney cortex mitochondria. For the 50% inhibition of Call accumulation, 2 × 10-4 M verapamil concentration was required in the presence of ATP (2 mM) and phosphate (5 mM). Omission of phosphate from the medium increased the inhibitory effect of verapamil on Ca2+ accumulation. Verapamil had no effect on Ca2+ accumulation in the presence of both ATP and succinate (7.8 mM), but further addition of phosphate resulted in a significant inhibition of Ca2+ accumulation by verapamil. Mg2+ accumulation of mitochondria was similarly depressed by verapamil. The same tendency was found as for the modification of verapamil effect by acetate in mitochondrial Ca2+ and Mg2+ accumulation. Succinate oxidation of mitochondria was not affected by verapamil in the absence of phosphate, but was inhibited by verapamil in the presence of phosphate. Therefore, it seemed reasonable to assume that the depression of Ca2+ and Mg2+ transport of mitochondria by verapamil is modulated by permeant anions. © 1979, The Japanese Pharmacological Society. All rights reserved.

引用

页码：745 / 751

页数：7

共 16 条

[1]

Fleckenstein A., Grun G., Reversible blockade of excitation-contraction coupling in rat's uterine smooth muscle by means of organic calcium antagonists (Iproveratril, D600, Prenylamine), Pflügers Arch., 307, R26, (1969)

[2]

Nayler W.G., Szeto J., Effect of verapamil on contractility, oxygen utilization and calcium exchangeability in mammalian heart muscle, Cardiovascular Res., 6, pp. 120-128, (1972)

[3]

Martin B.R., Clausen T., Gliemann J., Relationships between the exchange of calcium and phosphate in isolated fat-cells, Biochem. J., 152, pp. 121-129, (1975)

[4]

Wrobel J., Michalska L., The effect of verapamil on intestinal calcium transport, Europ. J. Pharmacol., 45, pp. 385-387, (1977)

[5]

Abe Y., Yukimura T., Iwao H., Mori N., Nakamura J., Okahara T., Yamamoto K., Effects of EDTA and verapamil in dogs, Japan. J. Pharmacol, 28, (1978)

[6]

Borle A.B., Regulation of cellular calcium metabolism and calcium transport by calcitonin, J. Membrane Biol., 21, pp. 125-146, (1975)

[7]

Gemba M., Effect of diuretics on ion transport of kidney cortex mitochondria, Japan. J. Pharmacol., 24, pp. 213-218, (1974)

[8]

Schnaitman C., Greenawalt J.W., Enzymatic properties of the inner and outer membranes of rat liver mitochondria, J. cell Biol, 38, pp. 158-175, (1968)

[9]

Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., Protein measurement with the Folin phenol reagent, J. biol. Chem., 193, pp. 265-275, (1951)

[10]

Chappell J.B., Crofts A.R., Calcium ion accumulation and volume changes of isolated liver mitochondria, Biochem. J., 95, pp. 378-386, (1965)

← 1 2 →