FLUORESCENCE PROPERTIES OF ANTHRYLVINYL LIPID PROBES

被引:28
作者
JOHANSSON, LBA [1 ]
MOLOTKOVSKY, JG [1 ]
BERGELSON, LD [1 ]
机构
[1] MM SHEMYAKIN BIOORGAN CHEM INST, MOSCOW 117988, USSR
关键词
anthrylvinyl lipid probes; fluorescence lifetime; fluorescent anisotropy; radiative lifetime;
D O I
10.1016/0009-3084(90)90044-R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report on the photophysical and fluorescence depolarization properties of the lipid probes 12-(9-anthryl)-11-trans-dodecenoic acid (AA), 1-acyl-2-[12-(9-anthryl)-11-trans-dodecenoyl]-sn-glycero-3-phosphocholine (APC) and N-[12-(9-anthryl)-11-trans-dodecenoyl] sphingosine-1-phosphocholine (ASM) in solvents and lipid vesicles. The fluorescence quantum yield (Φf) and lifetime (τf) were determined in aerated and degassed ethanol. Φf and τf are 0.59 and 7.1 ns and 0.87 and 10.0 ns, respectively for AA, APC and ASM. The radiative lifetime of 11.8 ± 0.3 ns, as calculated from Φf and τf is in good agreement with that of 12.3 ns predicted by the Strickler-Berg equation. The decay of the photophysics in viscous solvents is non-exponential and depends on the emission wavelength (λem), but becomes monoexponential and independent of λem at about 200 K. However, the emissionanisotropy remains dependent of λem below 200 K. This strongly suggests that the direction of the transition dipole moment changes along the fluorescence vibronic progression. As probe molecules in depolarization studies, anthracene; and anthrylvinyl derivatives should be used with care. © 1990.
引用
收藏
页码:185 / 189
页数:5
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