IMMUNOCHEMICAL DETERMINATION OF LIPOPROTEIN LP(A) - COMPARISON OF LAURELL ELECTROPHORESIS AND ELISA

被引：17

作者：

KOSTNER, GM ^{[1
]}

GRIES, A ^{[1
]}

POMETTA, M ^{[1
]}

MOLINARI, E ^{[1
]}

PICHLER, P ^{[1
]}

AICHER, H ^{[1
]}

机构：

[1] IMMUNO FORSCH LAB,VIENNA,AUSTRIA

来源：

CLINICA CHIMICA ACTA | 1990年 / 188卷 / 03期

关键词：

Lp(a) plasma concentration; Male/female differences; Pathological range;

D O I：

10.1016/0009-8981(90)90200-C

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The lipoprotein Lp(a) concentrations in serum from 520 persons (317 men and 203 women) were measured by two different Laurell electrophoresis assays (one in house and one commercial) and by an ELISA technique, using polyclonal antibodies from two different animal species. The following results were obtained. 1. The two Laurell techniques gave similar results. The mean values of all three methods were comparable, whereas median values obtained by ELISA were markedly lower (0.85, 0.97 and 0.057 g/l for the two Laurell assays and the ELISA, respectively). 2. All three methods correlated very well with a correlation coefficient of > 0.95. 3. Women had significantly higher values than men (p < 0.01). It was concluded that Lp(a) serum concentrations in the 'pathological range' could be measured with high enough precision by Laurell electrophoresis and by ELISA using polyclonal antibodies from different animal species. © 1990.

引用

页码：187 / 192

页数：6

共 18 条

[1] ENZYME-LINKED IMMUNOSORBENT-ASSAY OF LIPOPROTEIN(A) IN SERUM AND CORD BLOOD [J].