CONSTRUCTION OF BROAD-HOST-RANGE VECTORS FOR THE SELECTION OF DIVERGENT PROMOTERS

被引：24

作者：

RONALD, SL ^{[1
]}

KROPINSKI, AM ^{[1
]}

FARINHA, MA ^{[1
]}

机构：

[1] QUEENS UNIV,DEPT MICROBIOL & IMMUNOL,KINGSTON K7L 3N6,ONTARIO,CANADA

来源：

GENE | 1990年 / 90卷 / 01期

关键词：

alkaline phosphatase; Escherichia coli; luciferase; multiple cloning site; phage D3; plasmids pBR322 and pRO1614; promoter; Pseudomonas aeruginosa; Recombinant DNA; reporter gene; tetradecanal; β-galactosidase;

D O I：

10.1016/0378-1119(90)90451-V

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A series of promoter-probe plasmid vectors has been constructed which allows for the selection of DNA sequences containing divergent control elements. Each vector contains a pair of promoterless genes [encoding β-galactosidase (lacZ), alkaline phosphatase (phoA), and bacterial luciferase (luxAB)] arranged in an antiparallel fashion and separated by a large intervening multiple cloning site. The vectors permit direct detection of promoter activity on indicator plates after transformation. Cloned promoters are selected based on production of coloured products in the case of lacZ and phoA, and by the emission of light in the case of luxAB. These vectors have been tested using known divergent promoter elements from pBR322 and Pseudomonas phage D3. © 1990.

引用

页码：145 / 148

页数：4

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