CHARACTERIZATION OF DIVERGENT NTRA-DEPENDENT PROMOTERS IN THE ANAEROBICALLY EXPRESSED GENE-CLUSTER CODING FOR HYDROGENASE 3 COMPONENTS OF ESCHERICHIA-COLI

被引：49

作者：

LUTZ, S ^{[1
]}

BOHM, R ^{[1
]}

BEIER, A ^{[1
]}

BOCK, A ^{[1
]}

机构：

[1] UNIV MUNICH,LEHRSTUHL MIKROBIOL,MARIA WARD STR 1A,W-8000 MUNICH 19,GERMANY

来源：

MOLECULAR MICROBIOLOGY | 1990年 / 4卷 / 01期

关键词：

D O I：

10.1111/j.1365-2958.1990.tb02010.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The regulatory region of two divergently oriented transcriptional units involved in the formation of the gas‐evolving hydrogenase (isoenzyme 3) of Escherichia coli was investigated. DNA sequence analysis revealed the existence of a 210bp non‐coding region containing two sequences showing homology to ‐24/‐12 NtrA‐dependent promoters. These sequences were arranged in a divergent orientation entirety consistent with their being involved in transcribing the divergent operons. Through S1 protection experiments it could be shown that transcription of both promoters was NtrA‐dependent and that it was regulated in an identical manner: oxygen repressed expression, as did anaerobic growth in the presence of nitrate; transcription was induced in cells grown anaerobically in the absence of exogenous electron acceptors and formate was found to be obligately required for this anaerobic induction. Lying at an approximately equal distance between both promoters was a short stretch of DNA which showed similarity to the sequence previously identified (Birkmann and Böck, 1989a) as being necessary for formate induction of the fdhF gene. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：13 / 20

页数：8

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