CONVERSION OF DIHOMO-GAMMA-LINOLENIC ACID TO MONOHYDROXY AND DIHYDROXY ACIDS BY POTATO LIPOXYGENASE - EVIDENCE FOR THE FORMATION OF 8,9-LEUKOTRIENE-A3

被引：5

作者：

REDDY, CC ^{[1
]}

BERTLER, C ^{[1
]}

HAMMARSTROM, S ^{[1
]}

机构：

[1] LINKOPING UNIV, FAC HLTH SCI, DEPT CELL BIOL, DIV MED & PHYSIOL CHEM, S-58185 LINKOPING, SWEDEN

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1990年 / 279卷 / 02期

关键词：

D O I：

10.1016/0003-9861(90)90483-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Evidence for the formation of a positional isomer of leukotriene (LT) C3 (8,9-LTC3) from dihomo-γ-linolenic acid has been published (Hammarström, S. J. Biol. Chem. 256, 7712-7714, 1981). This report describes the conversion of dihomo-γ-linolenic acid to a postulated intermediate in former reaction, 8,9-LTA3, by purified lipoxygenase from potato tubers. 8(S)-Hydroperoxyeicosatrienoic acid (8(S)-HPETrE) was the most abundant dioxygenation product formed followed by 11-, 15-, and 12-HPETrEs (in decreasing order of abundance). In addition, 8(S),15(S)- plus 8(S), 15(R)-dihydroperoxyeicosatetraenoic acid (DiHPETrE) (EZE), and 8(S),15(S)- plus 8(S),15(R)-dihydroxy-eicosatetraenoic acid (DiHETrE) (EEE) were generated. Under anaerobic conditions only the latter two isomers of 8,15-DiHETrE (EEE) were obtained from 8-HPETrE. The results suggest that 8,9-LTA3 is synthesized by the sequential action of 8- and 11-lipoxygenase activities associated with the potato enzyme. © 1990.

引用

页码：211 / 217

页数：7

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