RAPID AND SIMPLE ISOLATION OF MULTIGRAM GOAT IGG FROM SERUM USING AVID AL AND RADIAL FLOW COLUMN

Multigram quantities (2.5-10 g) of highly purified IgG were obtained within 4 h from serum by using Avid AL(TM) packed in a radial-flow column. Avid AL(TM) is an affinity gel containing a synthetic, low-mol-wt ligand capable of selectively binding IgG from serum of all animal species tested. By packing the gel in a radial-flow column up to 500 mL, a high flow rate of 50 mL/min can be achieved without adversely affecting the performance of the gel and the purity of the isolated antibody.