A PROTEIN-KINASE GENE COMPLEMENTS THE LYTIC PHENOTYPE OF SACCHAROMYCES-CEREVISIAE LYT2 MUTANTS

被引:169
作者
TORRES, L
MARTIN, H
GARCIASAEZ, MI
ARROYO, J
MOLINA, M
SANCHEZ, M
NOMBELA, C
机构
[1] Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Madrid
关键词
D O I
10.1111/j.1365-2958.1991.tb01993.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
By genetic analysis of a thermosensitive autolytic mutant whose phenotype was complemented by osmotic stabilization with sorbitol, we identified gene LYT2 of Saccharomyces cerevisiae, which is probably involved in cell wall formation. A yeast gene complementing lyt2 strains was cloned and shown to carry an open reading frame coding for a 484-amino-acid protein exhibiting all the characteristic domains of serine/threonine protein kinases and highly homologous to other yeast protein kinases involved in control of the mitotic cycle. Mutants disrupted in the cloned gene also displayed an autolytic phenotype complemented by osmotic stabilization with sorbitol. However, genetic comparison of lyt2 mutants and disruptants of the protein kinase gene revealed that the cloned gene is not the structural gene LYT2 but a suppressor of the lytic phenotype, named gene SLT2, that was mapped to chromosome V. The product of gene SLT2 is the first protein kinase to be described in relation to the yeast cell-wall functions.
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页码:2845 / 2854
页数:10
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