SEPARATION OF CORTICOSTEROIDS AND AROMATIC-HYDROCARBONS BY CYCLODEXTRIN-MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY

被引：89

作者：

NISHI, H

MATSUO, M

机构：

[1] Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd., Yodogawa-ku, Osaka, 532, 16–89

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1991年 / 14卷 / 05期

关键词：

D O I：

10.1080/01483919108049298

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Micellar electrokinetic chromatography (MEKC) permits the separation of uncharged or electrically neutral compounds by the electrophoretic technique, but highly lipophilic compounds, e.g., corticosteroids and aromatic hydrocarbons could not be resolved by MEKC with sodium dodecyl sulfate (SDS) solutions because such solutes migrated with almost the same velocity as that of the SDS micelle, owing to their large micellar solubilization. The addition of cyclodextrins (CDs) to the SDS solution, that is, cyclodextrin-modified micellar electrokinetic chromatography (CD/MEKC), remarkably improved the resolution of these lipophilic compounds. The effects of concentration and the type of CDs on the separation of corticosteroids and aromatic hydrocarbons were investigated.

引用

页码：973 / 986

页数：14

共 20 条

[1] ALPHA-CYCLODEXTRINS, BETA-CYCLODEXTRINS AND GAMMA-CYCLODEXTRINS AS MOBILE PHASE ADDITIVES IN THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERIC COMPOUNDS .1. SEPARATION OF OPTICAL ISOMERS OF D,L-NORGESTREL [J].