STRUCTURE OF THE POLYSACCHARIDE CHAINS OF PSEUDOMONAS-PSEUDOMALLEI LIPOPOLYSACCHARIDES

The pathogenic bacterium Pseudomonas pseudomallei strain 57576 produces two partially O-acetylated O-antigenic polysaccharides (PS-I and PS-II). Methylation analysis and H-1 and C-13 NMR spectroscopy. including NOE experiments, showed PS-I to have the structure [GRAPHICS] and PS-II to have the structure [GRAPHICS] where 6d man Hep is the unusual higher sugar 6-deoxy-D-manno-heptose. PS-II is produced also by P. pseudomallei strains 100 and 110, and PS-I and O-deacetylated PS-II by strain 97.