EVOLUTIONARY CONSERVATION OF THE DYSTROPHIN CENTRAL ROD DOMAIN

被引：51

作者：

SHERRATT, TG ^{[1
]}

VULLIAMY, T ^{[1
]}

STRONG, PN ^{[1
]}

机构：

[1] HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DEPT HAEMATOL,LONDON W12 0NN,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1992年 / 287卷

关键词：

D O I：

10.1042/bj2870755

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dystrophin cDNA fragments encoding the C-terminal repeats of the central rod region have been expressed as fusion proteins. The polyclonal antisera raised to the purified fusion proteins have been characterized and neither antiserum cross-reacted with dystrophin-related protein. Antisera detected dystrophin with molecular mass close to that of the human in all terrestrial vertebrates and amphibia studied. Experiments with antisera to the N-terminal region of the dystrophin rod confirmed that epitopes to the rod region were conserved during this evolutionary period and the length of this domain remained unaltered.

引用

页码：755 / 759

页数：5

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