EFFECTS OF REACTIVE OXYGEN SPECIES ON ARACHIDONIC-ACID METABOLISM IN RABBIT PLATELETS

被引：20

作者：

SUMIYA, T ^{[1
]}

FUJIMOTO, Y ^{[1
]}

NISHIDA, H ^{[1
]}

MORIKAWA, Y ^{[1
]}

SAKUMA, S ^{[1
]}

FUJITA, T ^{[1
]}

机构：

[1] OSAKA UNIV PHARMACEUT SCI,DEPT HYG CHEM,MATSUBARA,OSAKA 580,JAPAN

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1993年 / 15卷 / 01期

关键词：

REACTIVE OXYGEN SPECIES; CYCLOOXYGENASE; LIPOXYGENASE; RABBIT PLATELETS; FREE RADICALS;

D O I：

10.1016/0891-5849(93)90129-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rabbit platelets were exposed to a reactive oxygen species (ROS) generating system (xanthine plus xanthine oxidase) to explore the effect of ROS on the formation of 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE), thromboxane (TX) B2, and 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) from exogenous arachidonic acid. Xanthine plus xanthine oxidase suppressed the production of 12-HETE, TXB2, and HHT by 65-69%. This effect was reversed by addition of catalase to the ROS generating system but not by superoxide dismutase, mannitol, or dimethylsulfoxide, indicating that H2O2 is the responsible metabolite. These results suggest that H2O2 plays an important role in the regulation of platelet cyclo-oxygenase and lipoxygenase activities.

引用

页码：101 / 104

页数：4

共 27 条

[1] HYDROGEN-PEROXIDE AND PLATELET-FUNCTION [J].

CANOSO, RT ;

RODVIEN, R ;

SCOON, K ;

LEVINE, PH .

BLOOD, 1974, 43 (05) :645-656

[2] HYDROGEN-PEROXIDE HAS A ROLE IN THE AGGREGATION OF HUMAN-PLATELETS [J].

DELPRINCIPE, D ;

MENICHELLI, A ;

DEMATTEIS, W ;

DICORPO, ML ;

DIGIULIO, S ;

FINAZZIAGRO, A .

FEBS LETTERS, 1985, 185 (01) :142-146

[3]

DUTILH CE, 1978, LIPIDS, V14, P241

[4]

EGAN RW, 1976, J BIOL CHEM, V251, P7329

[5]

FLOWER RJ, 1974, PHARMACOL RES COMMUN, V26, P33

[6]

FRIDOVICH I, 1970, J BIOL CHEM, V245, P4053

[7] THE INTERACTION BETWEEN LIPID-PEROXIDATION AND PROSTAGLANDIN SYNTHESIS IN RABBIT KIDNEY-MEDULLA SLICES [J].

FUJIMOTO, Y ;

TANIOKA, H ;

KESHI, I ;

FUJITA, T .

BIOCHEMICAL JOURNAL, 1983, 212 (01) :167-171

[8] EFFECT OF TERT-BUTYL HYDROPEROXIDE ON CYCLOOXYGENASE AND LIPOXYGENASE METABOLISM OF ARACHIDONIC-ACID IN RABBIT PLATELETS [J].

FUJIMOTO, Y ;

TAKAI, S ;

MATSUNO, K ;

SUMIYA, T ;

NISHIDA, H ;

SAKUMA, S ;

FUJITA, T .

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1992, 47 (04) :259-264

[9] EFFECTIVE USE OF RABBIT GASTRIC ANTRAL MUCOSAL SLICES IN PROSTAGLANDIN SYNTHESIS AND METABOLISM STUDIES [J].

FUJIMOTO, Y ;

NAKATANI, E ;

MINAMINO, H ;

TAKAHASHI, M ;

SAKUMA, S ;

FUJITA, T .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1044 (01) :65-69

[10] ANTIOXIDANT EFFECTS ON PROSTAGLANDIN SYNTHESIS IN RABBIT KIDNEY MEDULLA SLICES [J].

FUJITA, T ;

FUJIMOTO, Y ;

TANIOKA, H .

EXPERIENTIA, 1982, 38 (12) :1472-1472

← 1 2 3 →