IMMUNOFLUORESCENCE MICROSCOPY FOR THE RAPID DIAGNOSIS OF MELIOIDOSIS

被引：33

作者：

WALSH, AL

SMITH, MD

WUTHIEKANUN, V

SUPUTTAMONGKOL, Y

DESAKORN, V

CHAOWAGUL, W

WHITE, NJ

机构：

[1] SAPPASITPRASONG HOSP,DEPT MED,RATCHATANI,THAILAND

[2] JOHN RADCLIFFE HOSP,NUFFIELD DEPT CLIN MED,OXFORD OX3 9DU,ENGLAND

来源：

JOURNAL OF CLINICAL PATHOLOGY | 1994年 / 47卷 / 04期

基金：

英国惠康基金;

关键词：

D O I：

10.1136/jcp.47.4.377

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A direct immunofluorescent antibody test (DIF) was developed for the rapid diagnosis of melioidosis, a potentially fatal infection caused by Pseudomonas pseudomallei. In a clinical evaluation of 369 sputum, pus, or urine specimens from 272 patients with suspected melioidosis, the DIF had a sensitivity of 73% and a specificity of 99% compared with culture. Using this DIF, a confident diagnosis of melioidosis can now be made within two hours of admission to hospital, compared with the delay of two to four days required for culture results. Consequent early institution of specific antimicrobial therapy may help to save lives.

引用

收藏

页码：377 / 379

页数：3

相关论文

共 11 条

[1] MELIOIDOSIS - A MAJOR CAUSE OF COMMUNITY-ACQUIRED SEPTICEMIA IN NORTHEASTERN THAILAND [J].

CHAOWAGUL, W ;

WHITE, NJ ;

DANCE, DAB ;

WATTANAGOON, Y ;

NAIGOWIT, P ;

DAVIS, TME ;

LOOAREESUWAN, S ;

PITAKWATCHARA, N .

JOURNAL OF INFECTIOUS DISEASES, 1989, 159 (05) :890-899

[2]

Dance D. A. B., 1990, Reviews in Medical Microbiology, V1, P143

[3] IDENTIFICATION OF PSEUDOMONAS-PSEUDOMALLEI IN CLINICAL-PRACTICE - USE OF SIMPLE SCREENING-TESTS AND API20NE [J].

DANCE, DAB ;

WUTHIEKANUN, V ;

NAIGOWIT, P ;

WHITE, NJ .

JOURNAL OF CLINICAL PATHOLOGY, 1989, 42 (06) :645-648

[4] RAPID DIAGNOSIS OF MALARIA BY FLUORESCENCE MICROSCOPY WITH LIGHT-MICROSCOPE AND INTERFERENCE FILTER [J].

KAWAMOTO, F .

LANCET, 1991, 337 (8735) :200-202

[5]

LEELARASAMEE A, 1989, REV INFECT DIS, V11, P413

[6] A SENSITIVE METHOD OF DETECTING PROTEINS ON DOT AND WESTERN BLOTS USING A MONOCLONAL-ANTIBODY TO FITC [J].

SAMUEL, D ;

PATT, RJ ;

ABUKNESHA, RA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 107 (02) :217-224

[7] LATEX AGGLUTINATION-TEST FOR IDENTIFICATION OF PSEUDOMONAS-PSEUDOMALLEI [J].

SMITH, MD ;

WUTHIEKANUN, V ;

WALSH, AL ;

PITT, TL .

JOURNAL OF CLINICAL PATHOLOGY, 1993, 46 (04) :374-375

[8] MULTICENTER PROSPECTIVE RANDOMIZED TRIAL COMPARING CEFTAZIDIME PLUS COTRIMOXAZOLE WITH CHLORAMPHENICOL PLUS DOXYCYCLINE AND COTRIMOXAZOLE FOR TREATMENT OF SEVERE MELIOIDOSIS [J].

SOOKPRANEE, M ;

BOONMA, P ;

SUSAENGRAT, W ;

BHURIPANYO, K ;

PUNYAGUPTA, S .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1992, 36 (01) :158-162

[9] STAINING BACTERIAL SMEARS WITH FLUORESCENT ANTIBODY .2. RAPID DETECTION OF VARYING NUMBERS OF MALLEOMYCES-PSEUDOMALLEI IN CONTAMINATED MATERIALS AND INFECTED ANIMALS [J].

THOMASON, BM ;

MOODY, MD ;

GOLDMAN, M .

JOURNAL OF BACTERIOLOGY, 1956, 72 (03) :362-367

[10] HALVING OF MORTALITY OF SEVERE MELIOIDOSIS BY CEFTAZIDIME [J].

WHITE, NJ ;

CHAOWAGUL, W ;

WUTHIEKANUN, V ;

DANCE, DAB ;

WATTANAGOON, Y ;

PITAKWATCHARA, N .

LANCET, 1989, 2 (8665) :697-701