SUGAR NUCLEOTIDES AND NUCLEOTIDE-PEPTIDE COMPLEXES OF CHLORELLA PYRENOIDOSA - ISOLATION AND CHARACTERIZATION

The nucleotides of Chlorella pyrenoidosa were extracted with 80% hot ethanol and separated by fractionation on Dowex-1-formate. On the basis of chemical, enzymatic and chromatographic analysis, the following nucleotides were identified: NAD+, NADP+, 5′-CMP, 5′-UMP, 5′-AMP, 5′-GMP, 5′-IMP, UDP, ADP, ATP and thymidine oligonucleotides. The sugar nucleotides obtained were UDP-d-glucose, UDP-d-galactose, UDP-xylose, UDP-arabinose, UDP-N-acetylglucosamine, UDP-glucuronic acid, GDP-d-mannose and ADP-glucose. GDP-d-galactose and ADP-arabinose have not previously been found in green algae. Several nucleotide-peptide complexes, predominantly of adenine nucleotide, were also present. The properties of a derivative of ADP-ribose are discussed. Using 35S-labeled Chlorella cells, PAPS and sulfoquinovose were detected. The significance of these findings in relation to the metabolism of Chlorella is discussed. © 1970.