A FLUORIMETRIC ENZYMIC METHOD FOR ASSAY OF MIXTURES OF ORGANIC ACIDS

Rapid fluorimetric methods are described which involve six enzyme systems for the determination of mixtures of 21 organic acids. Lactate (Types II and IV), malate, glutamate, isocitrate, and β-hydroxybutyrate dehydrogenases are used, coupled with NAD, phenazine methosulfate and resazurin, in a fluorimetric procedure for the determination of acetic, adipic, benzilic, butyric, D-α - and D-β-hydroxybutyric, chloroacetic, DL-citric, formic, L-glutamic, glutaric, glycolic, threo-D-isocitric, L-lactic, L-malic, malonic, oxalic, phthalic, DL-succinic and L-tartaric acids in the approximate concentration range of 0.1-500 μg with an accuracy and precision of about 2%. The rate of production of the highly fluorescent resorufin is equated to the concentration of the acid. © 1969.