CHARACTERIZATION OF MAMMALIAN HEART ANNEXINS WITH SPECIAL REFERENCE TO CABP33 (ANNEXIN-V)

被引：43

作者：

PULA, G ^{[1
]}

BIANCHI, R ^{[1
]}

CECCARELLI, P ^{[1
]}

GIAMBANCO, I ^{[1
]}

DONATO, R ^{[1
]}

机构：

[1] UNIV PERUGIA,DEPT EXPTL MED & BIOCHEM SCI,ANAT SECT,CAS POST 81,SUCC 3,I-06100 PERUGIA,ITALY

来源：

FEBS LETTERS | 1990年 / 277卷 / 1-2期

关键词：

ANNEXIN; CALCIUM; MEMBRANE; BINDING; HEART;

D O I：

10.1016/0014-5793(90)80808-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porcine heart was observed to express annexins V (CaBP33) and VI in large amounts, and annexins III and IV in much smaller amounts. Annexin V (CaBP33) in porcine heart was examined in detail by immunochemistry. Homogenization and further processing of heart in the presence of EGTA resulted in the recovery of annexin V (CaBP33) in the cytosolic fraction and in an EGTA-resistant, Triton X-100-soluble fraction from cardiac membranes. Including Ca2+ in the homogenization medium resulted in a significant decrease in the annexin V (CaBP33) content of the cytosolic fraction with concomitant increase in the content of this protein in myofibrils, mitochrondria, the sarcoplasmic reticulum and the sarcolemma. The amount of annexin V (CaBP33) in each of these subfractions depended on the free Ca2+ concentration in the homogenizing medium. At the lowest free Ca2+ concentration tested, 0.8-mu-M, only the sarcolemma appeared to contain bound annexin V (CaBP33). Membrane-bound annexins V (CaBP33) and VI partitioned in two fractions, one EGTA-resistant and Triton X-100-extractable, and one Triton X-100-resistant and EGTA-extractable. Altogether, these data suggest that annexins V and VI are involved in the regulation of membrane-related processes.

引用

页码：53 / 58

页数：6

共 28 条

[1] ISOLATION AND CHARACTERIZATION OF 2 NOVEL CALCIUM-DEPENDENT PHOSPHOLIPID-BINDING PROTEINS FROM BOVINE LUNG [J].

BOUSTEAD, CM ;

WALKER, JH ;

GEISOW, MJ .

FEBS LETTERS, 1988, 233 (02) :233-238

[2] THE ANNEXIN FAMILY OF CALCIUM-BINDING PROTEINS [J].

BURGOYNE, RD ;

GEISOW, MJ .

CELL CALCIUM, 1989, 10 (01) :1-10

[3]

CAMPOSGONZALES R, 1988, EXP CELL RES, V184, P287

[4] DIVERSITY IN THE LIPOCORTIN CALPACTIN FAMILY [J].

CROMPTON, MR ;

MOSS, SE ;

CRUMPTON, MJ .

CELL, 1988, 55 (01) :1-3

[5] IDENTIFICATION OF 2 NOVEL ACIDIC, CALCIUM-BINDING PROTEINS IN BOVINE BRAIN [J].

DONATO, R ;

GIAMBANCO, I ;

AISA, MC ;

CECCARELLI, P ;

DIGERONIMO, G .

CELL BIOLOGY INTERNATIONAL REPORTS, 1988, 12 (07) :565-566

[6] 2 NOVEL BRAIN PROTEINS, CABP33 AND CABP37, ARE CALCIUM-DEPENDENT PHOSPHOLIPID-BINDING AND MEMBRANE-BINDING PROTEINS [J].

DONATO, R ;

GIAMBANCO, I ;

PULA, G ;

BIANCHI, R .

FEBS LETTERS, 1990, 262 (01) :72-76

[7] CALCIUM-SENSITIVE, LIPID-BINDING CYTOSKELETAL PROTEINS OF THE HUMAN PLACENTAL MICROVILLAR REGION [J].

EDWARDS, HC ;

BOOTH, AG .

JOURNAL OF CELL BIOLOGY, 1987, 105 (01) :303-311

[8] IDENTITY OF P36K PHOSPHORYLATED UPON ROUS-SARCOMA VIRUS TRANSFORMATION WITH A PROTEIN PURIFIED FROM BRUSH-BORDERS - CALCIUM-DEPENDENT BINDING TO NON-ERYTHROID SPECTRIN AND F-ACTIN [J].

GERKE, V ;

WEBER, K .

EMBO JOURNAL, 1984, 3 (01) :227-233

[9] INTERACTION OF 2 BRAIN ANNEXINS, CABP33 AND CABP37, WITH MEMBRANE-SKELETON PROTEINS [J].

GIAMBANCO, I ;

PULA, G ;

BIANCHI, R ;

DONATO, R .

FEBS LETTERS, 1990, 267 (01) :171-175

[10] CALPACTINS - 2 DISTINCT CA++-REGULATED PHOSPHOLIPID-BINDING AND ACTIN-BINDING PROTEINS ISOLATED FROM LUNG AND PLACENTA [J].

GLENNEY, JR ;

TACK, B ;

POWELL, MA .

JOURNAL OF CELL BIOLOGY, 1987, 104 (03) :503-511

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