PRESENCE OF 2 DNA-POLYMERASES IN TETRAHYMENA PYRIFORMIS

被引:17
作者
FURUKAWA, Y [1 ]
YAMADA, R [1 ]
KOHNO, M [1 ]
机构
[1] KYOTO UNIV, INST VIRUS RES, KYOTO 606, JAPAN
关键词
D O I
10.1093/nar/7.8.2387
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two DNA polymerases were detected in Tetrahymena pyriformis, strain GL. One (enzyme I) was sensitive to N-ethylmaleimide, while the other (enzyme II) was insensitive. The molecular weight of the enzymes, as determined by glycerol gradient centrifugation analysis, were approximately 130,000 and 70,000, respectively. Optimal concentration of MgCl2 was 10mM for enzyme I and 18mM for enzyme II. KCl inhibited enzyme I but stimulated enzyme II. Poly (dA-dT) served effectively as a template for enzyme I, while poly(dA). (dT) was an effective template for enzyme II.Enzyme I activity increased with cell growth and sharply declined after the cells reached the stationary phase. On the other hand, enzyme II activity appeared only at the end of log phase. In cells synchronized by starvation-refeeding technique enzyme I was markedly stimulated in correspondence to the rate of DNA synthesis, whereas the level of enzyme II activity changed to lesser extent. By ethidium bromide treatment, only enzyme I activity was induced. © 1979 Information Retrieval Limited.
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页码:2387 / 2398
页数:12
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