CANINE-DISTEMPER TERMINAL AND INTERGENIC NONPROTEIN CODING NUCLEOTIDE-SEQUENCES - COMPLETION OF THE ENTIRE CDV GENOME SEQUENCE

被引：82

作者：

SIDHU, MS

HUSAR, W

COOK, SD

DOWLING, PC

UDEM, SA

机构：

[1] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT MED,185 S ORANGE AVE,NEWARK,NJ 07103

[2] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT NEUROSCI,NEWARK,NJ 07103

[3] UNIV MED & DENT NEW JERSEY,NEW JERSEY MED SCH,DEPT MICROBIOL & MOLEC GENET,NEWARK,NJ 07103

[4] VET ADM MED CTR,NEUROL SERV,NEUROVIROL LAB,E ORANGE,NJ 07019

来源：

VIROLOGY | 1993年 / 193卷 / 01期

关键词：

D O I：

10.1006/viro.1993.1103

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sequences critical for the transcription and replication functions of canine distemper virus (CDV) RNA polymerase were analyzed. The sequence was obtained from polymerase chain reaction (PCR) products using either c-DNA clones from a genomic library as template or in most instances genomic CDV RNA. Clones coding for the precise 3’- and 5’-ends of the CDV genome were sequenced and the results confirmed by additional PCR experiments. The virtual identity of terminal sequences and spacing at the two noncoding ends speak to the importance of these areas in replication and transcription. The sequence for each of the CDV gene boundaries was defined and all were compared to related viruses. This report completes the sequence determination of the CDV genome. © 1993 Academic Press, Inc.

引用

页码：66 / 72

页数：7

共 23 条

[1] THE NUCLEOTIDE-SEQUENCE OF THE GENE ENCODING THE F-PROTEIN OF CANINE-DISTEMPER VIRUS - A COMPARISON OF THE DEDUCED AMINO-ACID-SEQUENCE WITH OTHER PARAMYXOVIRUSES [J].