DETECTION OF CGMP DEPENDENT PROTEIN-KINASE ISOZYMES BY SPECIFIC ANTIBODIES

被引：100

作者：

KEILBACH, A ^{[1
]}

RUTH, P ^{[1
]}

HOFMANN, F ^{[1
]}

机构：

[1] TECH UNIV MUNICH, INST PHARMACOL & TOXIKOL, BIEDERSTEINER STR 29, W-8000 MUNICH 40, GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1992年 / 208卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1992.tb17209.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two isozymes of cGMP-dependent protein kinase (cGMP kinase) have been identified. Polyclonal antibodies were developed which recognize both isozymes or specifically the I-alpha and I-beta isoform. The specificity of these antibodies was verified by using the recombinant or purified I-alpha and I-beta isozymes. The antibodies cross-reacted with the purified isozymes of cGMP kinase from bovine tracheal smooth muscle. The tissue concentration of cGMP kinase was determined by ELISA. High concentrations (> 10 pmol/g wet tissue) were present in bovine lung, rumen, trachea, aorta, uterus and stomach. The tissue distribution of the isozymes I-alpha and I-beta was investigated by immunoblots using crude extracts of the different tissues. The I-beta-specific antibody yielded strong signals with extracts of trachea, aorta, stomach and uterus, whereas heart, cerebellum and lung apparently contain mainly the I-alpha isozyme.

引用

页码：467 / 473

页数：7

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