THE INHIBITION OF HUMAN DUODENAL ADENYLATE-CYCLASE ACTIVITY BY CA2+ AND THE EFFECTS OF EGTA

被引：5

作者：

SMITH, JA ^{[1
]}

GRIFFIN, M ^{[1
]}

MIREYLEES, SE ^{[1
]}

LONG, RG ^{[1
]}

机构：

[1] NOTTINGHAM TRENT UNIV,DEPT LIFE SCI,NOTTINGHAM NG11 8NS,ENGLAND

来源：

FEBS LETTERS | 1993年 / 327卷 / 02期

关键词：

ADENYLATE CYCLASE; CALCIUM; EGTA; HUMAN DUODENUM;

D O I：

10.1016/0014-5793(93)80157-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study demonstrates that the inhibition of adenylate cyclase activity by Ca2+ is enhanced in the presence of increasing [EGTA] (0, 0.3, 1, 2.5 mM) by 2 orders of magnitude. It has been established that this effect is not because of poor Ca2+ buffering by low [EGTA] or high Ca2+ binding by the membrane preparation. It is present irrespective of stimulus. We suggest the enhanced sensitivity of adenylate cyclase to Ca2+ induced by EGTA is caused by the Ca-EGTA complex being a more inhibitory species than Ca2+. Thus consideration of the effects of the Ca-EGTA complex should be made when interpreting the results from experiments involving Ca2+ and EGTA.

引用

页码：137 / 140

页数：4

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