PURIFICATION OF EXOINULINASE AND ENDOINULINASE FROM CRUDE INULINASE EXTRACT FOR THE ANALYSIS OF FRUCTANS

被引：6

作者：

BAUMGARDNER, S

PRAZNIK, W

机构：

[1] Institute of Chemistry, Universität für Bodenkultur, 1180 Vienna

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 1995年 / 17卷 / 05期

关键词：

FRUCTANS; INULINASE; ELECTROPHORESIS;

D O I：

10.1016/0141-8130(95)98151-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes a technique for rapid and easy separation of a crude inulinase extract into exo- and endoinulinase from a commercially available inulinase stock (Novozym 230 from Novo Nordisk A/S) by means of non-denaturating polyacrylamide gel electrophoresis. The purified enzymes were tested with inulin. Characterization of the reaction products was done by high-performance thin-layer chromatography, size exclusion chromatography and semi-preparative reverse-phase high-performance liquid chromatography.

引用

收藏

页码：247 / 250

页数：4

相关论文

共 18 条

[1] PURIFICATION AND CHARACTERIZATION OF ENDO-INULINASE AND EXO-INULINASE [J].

AZHARI, R ;

SZLAK, AM ;

ILAN, E ;

SIDEMAN, S ;

LOTAN, N .

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1989, 11 (01) :105-117

[2] DETERMINATION OF INULASE ISOENZYMES ON POLYACRYLAMIDE GELS BY ACTIVITY STAINING [J].

BECK, RHF ;

PRAZNIK, W .

JOURNAL OF CHROMATOGRAPHY, 1986, 369 (01) :240-243

[3]

CHAUTARD P, 1981, ACTA MICROBIOL HUNG, V28, P245

[4] THE ACTION OF A HYDROLYTIC ENZYME SYSTEM FROM HELIANTHUS-TUBEROSUS L ON CARBOHYDRATES PRESENT IN THE TUBERS [J].

EDELMAN, J ;

BACON, JSD .

BIOCHEMICAL JOURNAL, 1951, 49 (04) :446-453

[5] METABOLISM OF FRUCTOSE POLYMERS IN PLANTS .4. BETA-FRUCTOFURANOSIDASES OF TUBERS OF HELIANTHUS TUBEROSUS L [J].

EDELMAN, J ;

JEFFORD, TG .

BIOCHEMICAL JOURNAL, 1964, 93 (01) :148-+

[6] ALCOHOL PRODUCTION FROM JERUSALEM ARTICHOKE USING YEASTS WITH INULINASE ACTIVITY [J].

GUIRAUD, JP ;

DAURELLES, J ;

GALZY, P .

BIOTECHNOLOGY AND BIOENGINEERING, 1981, 23 (07) :1461-1465

[7] INULIN HYDROLYSIS BY THE DEBARYOMYCES-PHAFFII INULINASE IMMOBILIZED ON DEAE CELLULOSE [J].

GUIRAUD, JP ;

DEMEULLE, S ;

GALZY, P .

BIOTECHNOLOGY LETTERS, 1981, 3 (12) :683-688

[8] STUDY OF INULINASE FROM CANDIDA-SALMENTICENSIS VAN UDEN AND BUCKLEY [J].

GUIRAUD, JP ;

VIARDGAUDIN, C ;

GALZY, P .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1980, 44 (06) :1245-1252

[9]

HEINZE B, 1991, J APPL POLYM SCI APP, V48, P207

[10] FRUCTOSE OLIGOSACCHARIDES IN MONOCOTYLEDONS - A POSSIBLE DELIMITATION OF THE ORDER LILIALES [J].

POLLARD, CJ .

BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 1982, 10 (03) :245-249